Biliverdin Reductase Protein (AA 3-296)

N° du produit ABIN666791

Cette protéine Recombinant Biliverdin Reductase est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Biliverdin Reductase Protein (AA 3-296) (ABIN666791)

Antigène: Biliverdin Reductase (BLVRA) (Biliverdin Reductase A (BLVRA))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Pureté: > 90 %

Détail du produit

Attributs du protein: AA 3-296

Séquence: MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK

Attributs du produit: Biliverdin reductase A, 3-296 aa, Human, Recombinant, E.coli

Purification: > 90% by SDS-PAGE

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 450pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of biliverdin of NADPH per minute at pH 8.8 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: Biliverdin Reductase (BLVRA) (Biliverdin Reductase A (BLVRA))

Autre désignation: Biliverdin Reductase A/BLVRA

Sujet: BLVRA, also known as biliverdin reductase A, belongs to the gfo/idh/mocA family. This protein is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. ( Bilirubin + NAD (P) + = biliverdin + NAD (P) H ) Recombinant BLVRA protein was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 33.3 kDa (295aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NCBI Accession: NP_000703